Sulfo-SMCC-sodium - Biochemical Assay Reagent - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESulfo-SMCC sodiumCat. No.: HY-D0975CAS No.: 92921-24-9分式: CHNNaOS分量: 436.37作靶点: Others作通路: Others储存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)溶解性数据体外实验 H2O : 6 mg/mL (13.75 mM; Need

2、ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.2916 mL 11.4582 mL 22.9163 mL5 mM 0.4583 mL 2.2916 mL 4.5833 mL10 mM 0.2292 mL 1.1458 mL 2.2916 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Sulfo-SMCC sodium种常的异双官能交联剂，其带有N-羟基琥珀酰亚胺 (NHS) 酯和马来酰亚胺基团，分别

3、与伯胺和巯基反应。体外研究 The crosslinker Sulfo-SMCC consists of a maleimide and an N-hydroxysuccinimide ester group to bind tosulfhydryl groups and primary amines, respectively. Sulfo-SMCC inhibits the end-to-end annealing ofstabilized Microtubules (MTs). MTs are treated with 250 M Sulfo-SMCC, and imaged after

4、 incubation for 0h, 6 h, and 24 h. MTs treated with Sulfo-SMCC shows a constant mean length, independent of the incubationtime 1.1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEPROTOCOLKinase Assay 1 Labeled and unlabeled porcine tubulin powders are used. Sulfo-SMCC is used. MTs are polymerized to

5、afinal concentration of 2 mg/mL in 80 mM PIPES buffer (pH 6.8) containing 10 M taxol, 2 mM MgCl2, 0.5 mMEGTA, and 1 mM GTP. A mixture of rhodamine-labeled tubulin and unlabeled tubulin (1:5) is used in thetime-dependent measurements. In these experiments, polymerized MTs are divided in 2 groups: con

6、trol andSulfo-SMCC (250 M) treated. Both samples are imaged after diluting 1:5 and incubating for 0 h (within 45minutes after polymerization), 6 h, and 24 h 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Prabhune M, et al. Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro. PLoS One. 2016 Aug 25;11(8):e0161623.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.Te