版权说明:本文档由用户提供并上传,收益归属内容提供方,若内容存在侵权,请进行举报或认领
文档简介
Strukturbiochemie中子散射2024/4/16Strukturbiochemie中子散射[1]Donnerstag,17:00–19:00Uhr10.05.200724.05.200707.06.200721.06.200705.07.2007->ExkursionzurGKSSTermineStrukturbiochemie中子散射[1]Neutronen-undRöntgenstreuung (gelösteSysteme)-3hFluoreszenzspektroskopie (verschiedeneVarianten)-2hSurfacePlasmonResonance-1hInfrarot-undCD-Spektroskopie-2hMassenspektroskopie-1hZetaPotential-1hThemenderVorlesung:Strukturbiochemie中子散射[1]AllgemeineVorbemerkungen:Strukturbiochemie-wasistdas?ErforschtdieStrukturvonProteinen,Nukleinsäuren,Lipiden,Zucker…..Komplexen(Ribosomen,Membranen,Zellen)…ErforschtdieFunktionundDynamikvonMolekülenUntersuchtdieWechselwirkungvonMolekülenStrukturbiochemie中子散射[1](Protein)strukturanalyseDiewichtigstenDatenbanken/pdb/home/home.doStrukturbiochemie中子散射[1]EMNMRStrukturbiochemie中子散射[1]/BLAST/Strukturbiochemie中子散射[1]/entrezStrukturbiochemie中子散射[1]/NDB/NDBATLAS/NucleicAcidDataBase/NDB/index.htmlStrukturbiochemie中子散射[1]URLfunktioniertgerademalnichtStrukturbiochemie中子散射[1]Strukturbiochemie中子散射[1]LiteraturGoogle/Wikipedia!Pingoud&C.Urbanke:ArbeitsmethodenderBiochemie,deGryuter(1997)
R.Winter&F.Noll:MethodenderbiophysikalischenChemie,Teubner(1998)
Internet:PubMed:ScienceDirect:
Strukturbiochemie中子散射[1]Grundlagen:WechselwirkungmitMaterieKontrastvariationAufbaueinesInstrumentesDatenaufnahmeundAuswertungStrukturanalyseanBeispielenNeutronen-undRöntgenstreuung(gelösteSysteme)
Strukturbiochemie中子散射[1]AccessableSizeRegimesatomicstructuremicellespolymersproteinsvirusbacterianeutrondiffractionX-raydiffractionelectrondiffractionSANSSAXSopticalmicroscopeelectronmicroscopeatomicforcemicroscopeLSSize/mScatteringMicroscopyStrukturbiochemie中子散射[1]IntroductiontoScatteringWhattypeofscatteringdoyouknow?electromagneticwavesLightscatteringX-Rayscatteringelectromagneticwaves–shortwavelengthNeutronscatteringparticles(Electronscattering)chargedparticlesHowdotheprobesinteract?OnlyElasticScattering!Strukturbiochemie中子散射[1]InelasticscatteringTransferofenergy/momentuminto/fromthesampleatomsProbesdynamics(structuralfluctuations)MainlyusedwithneutronsStrukturbiochemie中子散射[1]InteractionofRadiationwithMatterStrukturbiochemie中子散射[1]InteractionofRadiationwithMatterInteractionwithelectronsLightscatteringInteractionwithelectronsX-rayscatteringScattering‘strength’isproportionaltoZInteractionwithelectronspinpossibleInteractionwithnuclei(protonsandneutrons)NeutronscatteringScattering‘strength’doesnotvarysystematicallyInteractionwithnuclearspinpossibleInteractionwithelectronsandelectronspinpossibleStrukturbiochemie中子散射[1]AtomicScatteringfactors/lengthHR.Winter,F.Noll:MethodenderbiophysikalischenChemie,Teubner(1998)
X-RaysNeutrons
atomicmass/gmol-1Strukturbiochemie中子散射[1]ComparisonNeutron-andX-ray-scatteringlength
somerelevantelements[10-12cm]
n X-ray1H -0.37 0.282H 0.67 0.2812C 0.66 1.6814N 0.94 1.9616O 0.58 2.2431P 0.51 4.232S 0.28 4.4856Fe 0.95 6.72Strukturbiochemie中子散射[1]NeutronScatteringLength
ofbiologicalrelevantelements[10-12cm][F.Sears(1986),H.GlättliundM.Goldmann(1987)]X-RaySpeciality:AnomalousScattering(ASAXS)Strukturbiochemie中子散射[1] Neutrons X-RaysIntensity low highH-sensitivity high noneIsotope-sensitivity strong noneHeavyelements low highSpin-sensitivity strong averagePenetrationdepth high lowSamplesize/amount large smallMeasurementtime long shortInteractionwith nuclei electronshell electronshell unsystematic a
ZRadiationdamage none high
ToRemember:Strukturbiochemie中子散射[1]ToRemember:@H.Zabel,BochumStrukturbiochemie中子散射[1]Contrast
VariationWhenthemonstercame,Lola,likethepepperedmothandthearctichare,remainedmotionlessandundetected.Haroldofcourse,wasimmediatelydevoured.Strukturbiochemie中子散射[1]TheConceptofContrastVariationContrast=DifferenceofScatteringLengthDensitiesStrukturbiochemie中子散射[1]X-RayScatteringNeutronScatteringVolumeFractionD2OScatteringLengthDensityoftheSolvent[1010cm/cm3]ScatteringLengthDensityoftheSolute[1010cm-2]WaterSugarContrast=DifferenceofScatteringLengthDensitiesp(R)=rParticle(R)-rLM(R)p(R)=rParticle(R)-rLMr(R)=ScatteringLengthDensitiy=SumofScatteringLengthofallAtomsinaVolumeStrukturbiochemie中子散射[1]TheConceptofContrastVariationContrast=DifferenceofScatteringLengthDensitiesChangeofsolventcompositionChangeofsamplecomposition->IsotopicsubstitutionChangeofscatteringprocessUsually:CombinationofthemStrukturbiochemie中子散射[1]Examples:Polymer-SurfactantInteractionJuhaMertaandPerStenius:HelsinkiUniversityofTechnology,Espoo,FinlandVasilM.HaramusMaterials:SyntheticCationicPolymer:Poly(diallyldimethylammoniumchloride)–
PDADMACM=5–50
104,<M>~30104
AnionicSurfactant:Sodiumhexadecanoate,
NaPal
-C15H31COONa,CAC~0.1mM,CMC=1.6mM,Kraftpoint~62oCMotivation:Howdochargedpolymersinteractwithchargedsurfactants?'Application':forexamplemodelforprotein-surfactantorDNA-surfactantinteractionusedinpaperindustriestopurifycelluloseortoimprovepaperqualityFuture:softelectronics->organicswitchesHowarethetwocomponentsorganized?Strukturbiochemie中子散射[1]InternalContrastVariationC15H31COONa-NaPal(H)C15D31COONa-NaPal(D)SolventD2O-10-50510152025303540contrastintensityNaPal(H)NaPal(D)PDADMAC[1010cm-2][1020cm-4]WhereisthesurfactantNaPalandwherethepolymerPDADMAC?Examples:Polymer-SurfactantInteractionSurfactantStrukturbiochemie中子散射[1]HDPDADMAC/NaPal/D2Odifferenceinthedistributionofsurfactantandpolymerintheaggregatesonthelengthscaleof20-30Å.Examples:Polymer-SurfactantInteractionInternalContrastVariationStrukturbiochemie中子散射[1]Examples:Polymer-SurfactantInteractionConclusion:ShellStructureofPDADMAC/NaPal:SurfactantCore&PolymereShellHDDmax=55Å,RCS,gNaPal(H)-16±1ÅNaPal(D)-24±1ÅJ.Merta,V.M.Garamus,R.Willumeit,P.Stenius(2002)Langmuir,18,7272-7278DistanceDistributionStrukturbiochemie中子散射[1]CompositionofbacterialribosomesMass:2.4×106DaDiameter:270ÅMass:1.5×106DaDiameter:230ÅMass:0.9×106DaDiameter:200Å[R.JünemannundK.H.Nierhaus:NaturwissenschaftlicheRundschau
10(1993)391-400]Strukturbiochemie中子散射[1]D.I.Svergunetal.:JMB271(1997)602-618RibosomeStructuresStrukturbiochemie中子散射[1]D.I.Svergunetal.:JMB271(1997)602-618RibosomeStructuresStrukturbiochemie中子散射[1]D.I.Svergunetal.:JMB271(1997)602-618ShapeandRNA-distributionModellingRibosomeStructuresStrukturbiochemie中子散射[1]NeutronScatteringLength
ofbiologicalrelevant
温馨提示
- 1. 本站所有资源如无特殊说明,都需要本地电脑安装OFFICE2007和PDF阅读器。图纸软件为CAD,CAXA,PROE,UG,SolidWorks等.压缩文件请下载最新的WinRAR软件解压。
- 2. 本站的文档不包含任何第三方提供的附件图纸等,如果需要附件,请联系上传者。文件的所有权益归上传用户所有。
- 3. 本站RAR压缩包中若带图纸,网页内容里面会有图纸预览,若没有图纸预览就没有图纸。
- 4. 未经权益所有人同意不得将文件中的内容挪作商业或盈利用途。
- 5. 人人文库网仅提供信息存储空间,仅对用户上传内容的表现方式做保护处理,对用户上传分享的文档内容本身不做任何修改或编辑,并不能对任何下载内容负责。
- 6. 下载文件中如有侵权或不适当内容,请与我们联系,我们立即纠正。
- 7. 本站不保证下载资源的准确性、安全性和完整性, 同时也不承担用户因使用这些下载资源对自己和他人造成任何形式的伤害或损失。
最新文档
- 激发数字产业发展活力实施方案
- 固体饮料行业投资机会分析报告
- 2024年植树节义务植树活动方案8篇
- 2024产品包销合同
- 2024劳务派遣用工安全简单协议书范本
- 三、车身覆盖件修复
- 【可行性报告】2023年船舶制造行业项目可行性分析报告
- 【可行性报告】2023年聚砜PSF项目可行性研究分析报告
- 2023年氧化铁黄相关行业项目成效实现方案
- 通信安全员ABC证考试题库及答案
- 餐券模板完整
- 大学生跨文化交际能力的现状调查和对策研究
- X射线荧光光谱理论考试考试题库(选择题)
- 信息检索与利用智慧树知到课后章节答案2023年下石河子大学
- 基于项目式学习的中小学创新教育实践
- 跨文化认知与文明互鉴:埃及智慧树知到课后章节答案2023年下福州大学
- 中国华电集团公司成员单位名单
- 知名国企建筑公司物资采购及管理制度
- DB32T 4340-2022 沥青红外光谱法相似度识别与SBS含量试验检测规程
- 2023年大学德语四级真题及详解【圣才出品】
- (完整版)康复治疗知情同意书
评论
0/150
提交评论