肠道感染病毒精品推荐课件

荆 案 酥 混 绊 唯 贩 挟 谐 民 障 榜 妨 桨 说 笔 周 涸 震 贴 拟 兰 胞 般 曾 炳 出 打 隘 擦 脯 挫 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 Chapter 21 Human enterovirusHuman enterovirus Viruses of causing acute gastroenteritisViruses of causing acute gastroenteritis 侠 丛 坠 符 糜 蕉 炽 讯 捷 洪 筏 茎 哪 碾 夏 膛 琴 泥 姨 誊 挺 恼 企 暇 硷 白 伊 孺 爪 壁 颓 藩 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 PoliovirusPoliovirus CoxsackievirusCoxsackievirus group A : types 122, 24 group B : types 16 Enteric cytopathogenic human Enteric cytopathogenic human orphan(ECHO) virus orphan(ECHO) virus New enterovirusesNew enteroviruses (types 13) (31 types) (types 6871) 喧 晌 赴 倔 折 爆 撵 幂 堑 铭 井 母 族 屉 莲 涌 尤 哇 辜 疮 卢 煞 枷 覆 桥 坝 斩 瓦 疗 纳 伦 骂 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 of enterovirusof enterovirus Shape and structureShape and structure d=2430 nm, spherical and naked virus with icosahedral nucleocapsid polyproteinpolyprotein VP1VP4 and functional proteinVP1VP4 and functional protein 衣壳蛋白衣壳蛋白(VP1(VP1与受体结合与受体结合) ) genome:+ssRNA 1.Biological properties1.Biological properties 弯 公 柴 澜 纯 酶 煤 障 叛 弗 绰 哭 栓 凤 相 梭 曙 呢 韩 典 走 临 屯 栋 垄 御 酒 膛 钾 傈 酥 纳 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 Cultivation RresistanceRresistance(stronger) (pH39(pH39稳定稳定, ,污水和粪便中可存活数月污水和粪便中可存活数月) ) Type-specific antigenType-specific antigen D(density)/N(native) antigen C (coreless) antigen primary cell culture-CPE animal inoculation (A1,A19,A22) 哨 沁 瘟 股 课 财 噪 缘 寓 筷 乡 暮 传 哼 睡 鼻 服 汁 酉 揩 嗡 启 娇 卵 舜 厕 峰 婶 淳 妻 沧 投 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 2.2.Pathogenicity of enterovirusesPathogenicity of enteroviruses Replicate in the intestinal tract and Replicate in the intestinal tract and spread through bloodstream to target spread through bloodstream to target organsorgans Via fecal-oral route cause a variety Via fecal-oral route cause a variety of diseases or syndromes of diseases or syndromes (夏秋季为主要流行季节) 不同肠道病毒可引起相同的临床症状,同一种 病毒可引起几种不同的临床疾病 P275276表 (通常有二次病毒血症) -from subclinical infection to severe infection of CNS 吝 辐 验 既 珊 袄 粉 贞 疏 滋 茨 竿 晶 弊 斟 背 炭 拎 涤 琐 榆 摈 探 宋 块 熬 歉 处 铃 情 玩 迈 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 source of infectionsource of infection (patients and carrier) cause poliomyelitis by fecal-oral routecause poliomyelitis by fecal-oral route clinical findings of poliomyelitisclinical findings of poliomyelitis Poliovirus (neurotropic virus) *subclinical infection(90%) *abortive infection (5%) *nonparalytic poliomyelitis/aseptic meningitis(1-2%) *paralytic poliomyelitis(0.1%-2%) nonparalytic : paralytic=1001000 : 1nonparalytic : paralytic=1001000 : 1 -pathogen of poliomyelitis-pathogen of poliomyelitis(脊髓灰质炎或小儿麻痹症) 鬼 沈 饭 诗 波 韧 炯 违 菊 副 弯 忍 纺 钙 嘱 懂 董 琐 孙 滑 呆 寡 赊 摊 仔 坎 偶 堆 赴 薯 惫 动 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 Coxsackievirus and ECHO virusCoxsackievirus and ECHO virus Enterovirus 71Enterovirus 71 -cause a variety of diseases or -cause a variety of diseases or syndromessyndromes (such as aseptic meningitis and encephalitis and so.on) (P275276P275276表表) ) initial site of infection and route of transmission E70,CA24 and Ad3,7AHC (急性出血性结膜炎) -The main cause of acute The main cause of acute hemorrhagic conjunctivitis (AHC)hemorrhagic conjunctivitis (AHC) 暗 磁 密 副 呕 恨 舶 绍 粗 钉 浆 啊 吝 锦 供 拙 茂 立 惯 抒 渣 琳 谬 余 熊 阶 世 馏 釜 侦 唤 丈 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 Enterovirus 71 Enterovirus 71 - lifelong type-specific immunity ( neutralizing antibody ) -causecause viral CNS diseaseviral CNS disease (such as aseptic meningitis and encephalitis and so.on) and HFMDHFMD(hand-foot-and-mouth disease,手足口病) 3.3.ImmunityImmunity of enterovirusesof enteroviruses (neurotropic virus) 表 幢 雾 迫 录 缔 玫 冶 僻 标 叙 遗 戴 三 噬 垣 啥 偶 予 捕 板 售 汽 慈 撵 萌 措 辈 珍 拆 分 韦 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 4.4.Laboratory diagnosis Laboratory diagnosis of enteroviruses of enteroviruses Isolation and identification of virus Isolation and identification of virus Detection of viral antibody and RNADetection of viral antibody and RNA 5.5. Specific prevention of Specific prevention of poliovirus Artificial active immunity Artificial passive immunity IPV ( Salk vaccine) IPV ( Salk vaccine) OPV(Sabin vaccine ) OPV(Sabin vaccine ) 均为三价混合疫苗；但均为三价混合疫苗；但 OPVOPV与疫苗相关麻痹型脊髓与疫苗相关麻痹型脊髓 灰质炎灰质炎(VAPP)(VAPP)发生有关发生有关 (CPE)(CPE) 蕴 嫉 瑞 居 酣 诧 篓 性 失 廊 狞 闰 职 搜 段 仇 桐 炔 较 怒 恶 账 估 爆 浆 蝶 彦 渗 咽 菏 叼 鹃 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 -Viruses of causing -Viruses of causing acute gastroenteritis acute gastroenteritis Rotavirus (RV)Rotavirus (RV) OOther acute gastroenteritis virus ther acute gastroenteritis virus 击 鄂 疼 克 蕴 疵 怂 乃 限 弛 吻 石 讨 协 甜 累 炳 赫 玄 植 贵 蚀 甫 疯 窍 败 咐 属 孩 动 泄 通 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 1. 1.Shape of virionShape of virion -d=60-80nm, spheric and naked virus with double-shelled capsid 2.Genome 2.Genome a appearance of a “wheel”ppearance of a “wheel” ( (轮状病毒轮状病毒) ) consists of 11 segments of ds-RNA code for 6 structural proteins(VP1-4,6,7) and 5 non-structural proteins (NSP1NSP5) 蚕 结 符 呻 迢 爵 嗡 者 兢 贫 虚 棱 侥 混 徐 军 底 朵 绦 欺 叶 战 株 殿 承 详 萄 汉 耻 绢 祟 尊 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 3.Pathogenicity3.Pathogenicity Source of infection Source of infection Route of transmission Route of transmission The main The main viral viral cause cause of of acute gastroenteritis acute gastroenteritis group A: main pathogen of acute gastroenteritis in infants group B cause epidemic diarrhea in adult group C: cause human or animal sporadic diarrhea (or ADRV)(or ADRV) (6(6个月个月22岁岁) ) 牌 伪 坏 镇 鸣 矩 熏 驮 固 途 柏 痕 俭 扫 垫 栅 氦 握 苞 篷 器 艰 颠 龙 禹 拼 尺 愈 喘 仪 球 玩 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 4. 4. Diagnosis of infectionDiagnosis of infection Detection of viral particle Detection of viral particle and viral antigen and viral antigen (EM,ELISA) PAGE & RT-PCRPAGE & RT-PCR (聚丙烯酰胺凝胶电泳) 拙 诫 静 沤 镇 酮 恍 罗 玲 房 跺 肇 俄 留 超 烩 分 雾 捷 倾 缓 憾 躁 授 食 拄 似 乒 跨 稍 洼 疙 0 8 肠 道 感 染 病 毒 0 8 肠 道 感 染 病 毒 Including:Including: Enteric adenovirusEnteric adenovirus C