毕业学生菌种保藏.doc

高温PHAGE及栖热菌 2008.4.16洱源0805h Thermus(Vs)洱源0805+洱源0805h Thermus(Vs)洱源0805+洱源0805h Thermus(Vs)洱源0805+洱源0805h Thermus(Vs)洱源0805+1、 Thermus(Vs)MZ0834HMZ23HMZ34HMZ29#HMZ29HMZ混合洱源0805+h4Bah25HMZ0829HMZ0834+MZ0834HMZ23HMZ34HMZ29#HMZ29HMZ混合洱源0805+h4Bah25HMZ0829HMZ0834+MZ0834HMZ23HMZ34HMZ29#HMZ29HMZ混合洱源0805+h4Bah25HMZ0829HMZ0834+2、 洪伟 硫化叶菌黄瓜2#-3黄瓜1#-3NJ36#HMZ9-6DGG2#-2TYQ1#-3黄瓜2#-3黄瓜1#-3NJ36#HMZ9-6DGG2#-2TYQ1#-3黄瓜2#-3黄瓜1#-3NJ36#HMZ9-6DGG2#-2TYQ1#-33、 洪伟ThermusEY0805EY0806EY0807EY0802EY0818NJ0821NJ0807NJ0810NJ0806NJ0817NJ0801NJ0804NJ0815NJ0840RH8013NJ0814NJ0838NJ0802NJ0811NJ0835NJ0812EY0805EY0806EY0807EY0802EY0818NJ0821NJ0807NJ0810NJ0806NJ0817NJ0801NJ0804NJ0815NJ0840RH8013NJ0814NJ0838NJ0802NJ0811NJ0835NJ0812EY0805EY0806EY0807EY0802EY0818NJ0821NJ0807NJ0810NJ0806NJ0817NJ0801NJ0804NJ0815NJ0840RH8013NJ0814NJ0838NJ0802NJ0811NJ0835NJ08124、 HW 2009.7.12 绿色TG7H22P1+P2EY0805TC16+P2TG17H4H1917SP1TG1612SMH7TG7H22P1+P2EY0805TC16+P2TG17H4H1917SP1TG1612SMH7TG7H22P1+P2EY0805TC16+P2TG17H4H1917SP1TG1612SMH76、2008.11.3方盒HMZ29h+HMZ23h+HMZ34h+NJ混合FG富集TYQ混合富集HMZ混合富集BLZ混合富集HMZ29hEY0805 hHMZ34hHMZ34hXNJ16XNJ16hTC16B4(3)G2TC16B2(3)G4TC16B4(1)G3TC16B1(3)G2TC16B4(3)G2TC16B6(1)G2TC16B4(2)G2TC16B7(1)G2TC16B7(3)G2TC16B4(3)G2TC16B5(1)G2TC16B7(2)G2TC16B31)G2TC16B1(1)G2TC16B2(1)G2TC16B1(2)G2TC16B6(1)G2TC16B2G1TC16B6G1TCH16B4G1TCH16B5G1TC16B3G1XNJ16h+TCH16B5G1TC4seqEY0805HMZ34TC4-17 HW 2008.10.9 方盒 北样品准备HMZ23#BFJJJK1h+斑Kkq1#hHMZ34BFJJTHMZ29BFJJTHMZ0829HMZ0834HMZ0823HMZ29HMZ34HMZ混合E080508170801HMZ23E0805HMZ23#BFJJJK1h+斑Kkq1#hHMZ34BFJJTHMZ29BFJJTHMZ0829HMZ0834HMZ0823HMZ29HMZ34HMZ混合E080508170801HMZ23E0805HMZ23#BFJJJK1h+斑Kkq1#hHMZ34BFJJTHMZ29BFJJTHMZ0829HMZ0834HMZ0823HMZ29HMZ34HMZ混合E080508170801HMZ23E0805、方盒KKQ富集KKQ富集KKQh1EY2h3KKQ1富集KKQ富集KKQ1富集KKQ新混合GMQh5EY2-2NJ菌KKQ混合GMQh4 EY2-1KKQ1+GMQh3EY1-6EY1-5GMQh2KKQ土壤富集GMQhGMQhEY1-EY1-EY2-hKKQ-2EY2-4EY2-5EY1-2EY2-1EY1h9、 HW2008.10.23 方盒栖热及hEY0806NJ0810NJ0821NJ0801NJ0848NJ0805RH0810NJ0852RH0802RH0812NJ0847HMZ0828HMZ0827NJ0815RH0816NJ0845RH0811NJ0801NJ0806NJ0814NJ0807NJ0806NJ0847NJ0840EY0805HMZ0801HMZ0831HMZ0833YXPHMZ0824HMZ0823HMZ0829HMZ0819HMZ0826HMZ0835NJ0815NJ0816HMZh7HMZ0807h+NJ0802RH0845NJH0803NJH0806NJH0805RH0811NJ0842NJ0846NJ084810、 HW 样品2008910方盒HMZ0819HMZ0823HMZ0816HMZ0824HMZ0825HMZ0822HMZ0829HMZ0834EY0805RH0811NJ0803NJ0854NJ08NJ08RH08NJ08080804NJ0858NJ08460809NJ0809NJ0842NJ0806EY0806NJ08NJ08RH08NJ0838NJ0859NJ0851NJ0843NJ0855NJ0850NJ0815HMZ0821HMZ0818HMZ0824NJ0802HMZ0825HMZ0822RH0807HMZ0819HMZ0820RH0808HMZ0819NJ0811NJ0812NJ083511、HWLQP2009栖热菌及200932519+P19+P22S+P43DM4M14H16BC19H17H18DM16DMTC1610MLYC16DE116BLEY0805H12H22h4h16HCT10HCT43DM12、2009.4thermusphageEY0805TC16P1H17H4EY1805P1+P2NJTC1617SP2H22H18H12H714DS14DS12S14BMT2P2+H14AOSTC16P2TG1H1714BM14SM12SM13、HW200912.5DNAH4 HostH4H6P10H10P4亚17EY08054DNA基因亚171012TC414、LQP5185195205215225235305LC523-1523-2525-215、LQP2009326栖热菌HMZ39HMZ43HMZ34HMZ 29LJXQ116LJXQ124LJXQ110LJXQ134LJXQ122LJXQ114LJXQ119LJXQ110LJXQ1NJ253NJ217HMZ23HMZ2318HMZ7LJXQ12LJXQ6TC16LJXQ316HJTG17+TG17TG17TG14TG12TG07TG08TG05TG04TGYXR101003040708101213141516171816TG17TG1717、TG17TG216+HJ2XNJH19XNJH21TC16XNJH16XNJH11XNJH03XNJH 01XNJH04XNJH05XNJH07XNJH17XNJH14XNJH 20XNJH 06XNJH15XNJH 16HYSTZXQXM118、HJThermus2009113J1J2J3J4J5J6J7J8J9J10X2X3X4TC16TC16X1J7J4J5J3X2J8J10X4J119、HJTG1780TG2TG2TG2TG2TG2TG2TG17甘油TG17TG17DMSOTG17甘油TG17浓缩TG17TG17TG17DMSOTG17甘油TG17DMSOTG17TG17DMSOTG17DMSOTG17甘油TG17浓缩TG17TG17甘油TG17甘油20、LQP20101015高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤阴性克隆高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤高纤怀胎高纤高纤高纤高纤高纤高纤高纤高纤怀胎高纤怀胎怀胎怀胎怀胎高纤高纤怀胎阴性高纤高纤高纤高纤高纤高纤21、LPQ 亚subthemus菌种111213151617181921222324252627282911121315161718192122232425262728291112131516171819212223242526272829124568912456891245689注：1-mmy6-1 2-zy35 3-4-z 4-Ry25 5-Ry4-3 6-Zzcy17-1 7mmy4-18-Zzcy20-2 9-zzcy7-2 pigment from Eryuan 10-nothing 11-Eryuan1-512-WQBGR2-5 13-Eryuan7# 14-Eryuan6-3 15- WQBGR2-416- Eryuan1-4 17- WQBGR2-6 18-mmR2-1 19- WQBGR2-1 red pigment from Eryuan 21-RHR8-1 22- RHR12-1 23- RH17-1-1 24- RHR15-325- RHR8-2 26- RHR2-2 27- RHR15-5 28- RHR12-4 29- RHR12-3 red pigment from Rehai22、LQP5#175#165#155#145#135#125#115#105#95#85#75#65#55#45#35#25#15#175#165#155#135#125#115#105#95#85#75#65#55#45#35#25#1y2 黄4y2 黄4y2 黄45#305#295#285#275#265#255#245#235#225#215#205#195#185#175#165#155#145#135#125#115#105#95#85#75#65#55#45#35#25#1Tamy1223、LQP 2008、1、23高酪11高酪12高酪13高酪14高酪15高酪16高酪17高酪18高酪19高酪20高酪21高酪22高酪23高酪24高酪25高酪26高酪27高酪28高酪29高酪30高酪31高酪32高酪33高酪34高酪11高酪12高酪13高酪14高酪15高酪16高酪17高酪18高酪19高酪20高酪21高酪22高酪23高酪24高酪25高酪26高酪27高酪28高酪29高酪30高酪31高酪32高酪33高酪34高酪11高酪12高酪13高酪14高酪15高酪16高酪17高酪18高酪19高酪20高酪21高酪22高酪23高酪24高酪25高酪26高酪27高酪28高酪29高酪30高酪31高酪32高酪33高酪3424、mcs yaf 腾冲酸性富集培养物 2008.10.19Host8 yaf 11.10Host8 yaf12.4HMZ噬斑0807HMZ08斑07 03菌体Host8 yaf12.4Host8 yaf11.4HMZ08斑07 斑HMZ08斑07 斑Host8 yaf 11.10混合酸1混合酸1混合酸1混合酸9混合酸8混合酸7混合酸6混合酸5混合酸4混合酸3混合酸2混合酸125、HW 方盒 NJ0858 2G 8.21NJ0842Y+RH0806YNJ0844CNJ0852C8.21ANH4测序+CaL2 7-13NJ0811CYEY0805 hostXJ4PE斑 NJ0813CYRH0807YXJ0854CYNJ4富集菌体EY0805ZG8,21NJ混合富集菌NJ0808ZG8,218.21ARH0809YNJ0846YNJ0843YNH4测序+C9.13EY0805宿主+斑NH4测序+克隆Thermus斑清AXJ富集斑NJ0859YNJ富集XJ0857YXJ富集斑XJ富集菌NJ0850Y+NJ0857YNJ0855YNJH富斑NJ0816CYNJ0813CYEY0805斑Thermus斑清NJH富斑NJH富斑NJ0858ZG8,21RH0808YPEC2G斑RH0805YNJH富斑NJH富斑复苏NJ0848CNJ0843CNJHPEC斑NJ0805CYXJ0858CYRH0802CYEY0805ZGRH0805YNJHPEC斑NJ0851YNJ0805CYNJ0849Y+NJ0818CYNJ0820CNJ0851YRH0804YNJ0807YRH0810YNJ0816CY26、LQP RHA121ERICLR18质粒ERICTRBL21BL21EY0801高效胶回收AmpERIC21aKanaDH5aSau3AIPuc1181818Origan iBERIC2SauAIBL21EY0801质粒18#阳性克隆1818Origin.1B18HRBL21HFOrigin.1BCL.LTetMarkerBL21plyssBL21ROSSTTaBOSSTTaDH5aEY0801EY0801EY0802EY0801EY0801EY0801ROSSTTaEY0801EY0801EY080127、 LYQ 青色长方盒DGG质DSGX2LHG1-3HG1-2HG4ORF108b sense ORF138anti质粒质粒质D-1质Z2-3质HMZTYQXHT-质TYQ质7K质大泉B质TY混质70CF质3132333441424344515253547172737410110210310428、LYQTYQ#1-9去Ca2+TYQ#1-9去Ca2+TYQ#1-9去Ca2+TYQ#1-9去Ca2+TYQ#1-9无CaCO3TYQ#1-76#-2黄瓜4# 黄瓜4#小河边TYQ1#-7TYQ1#-7HMZ9#-6TYQ1#-7TYQ1#-7DGG2#-2DGG2#-2TYQ1#-7小河边TYQ1#-9黄瓜1#-3黄瓜1#-3DGG2#-4DGG2#-4TYQ1#-3黄瓜1#-3黄瓜1#-4黄瓜1#-2HMZ9#-6DGG2#-4HMZ9#-15小河边TYQ1#-3TYQ1#-3HMZ6#-2HMZ6#-2HMZ6#-2HMZ9#-6HMZ5#-9TYQ1#-10黄瓜1#-5黄瓜1#-5TYQ1#-10黄瓜1#-5黄瓜1#-5TYQ1#-1029、LYQ 硫化叶菌测序33774886699XHBAmp10Buffer(RCR)10mg/mlRNaseA10Buffer(RCR)DGGTaqDNA聚合酶TaqDNA聚合酶XHB30、-20 洪伟KM1 10-34 51056.23亚177.121010.15亚475.9126.214 15%甘油8.27亚1710107.1112裂解完全7.6EY080546.2EY0805TC16 UC11.24EY0805G126.21EY0805415%G08.2亚175.9P10 UC11.29EY0805UC 6.20P4+DNasel 11.29TC16EY0805EY080511.2610裂解完全7.6TG2UC45106亚171010亚177.12TG210UC4.2610GYTC164.26 UCEY0805EY08054 51076.515%甘油 亚17 8.274 6.2EY0805 11.260805 11.261014 UC 4.2512 UC 4.264 8109 6.11104裂解完全T1611074 6.30EY0805 5.28080514 5.11Host 1ZG 6.10 PUC118 10-6P10DNasel UC 09.11.9亚17亚17Ph14 UC 09.11.29亚17亚171010+10%DMSO 7.11EY0805未知 09.6.2114TC16 10%DMSO 09.8.314 9109 DMSO 6.11P10 09.11.29 UC亚1710G14 5.11TG21109 G 6.2TC169.1631、 HW -2012UC 5.12T4-1 08.12.170805 11.1310UCHMZ34#UC 11,14HMZ29UC 11.14XNJH16 UC 11.20TC16 UC 5,9UC08054UC 5.1210 UC 5,22HMZ23 7.716 11.1314赵UC 09.4.1614 UC 5,12UC0805陆UC 09.4.16h12 UC 6.20UC10TC16 UC 5.9P2 UC 09.4.1614 UC 5,